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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
04/09/2020 |
Actualizado : |
05/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CAFFARENA, D.; MEIRELES, M.V; CARRASCO-LETELIER, L.; PICASSO-RISSO, C.; SANTANA ,BN; RIET-CORREA, F.; GIANNITTI, F. |
Afiliación : |
RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; MARCELO VASCONCELOS MEIRELES, São Paulo State University Department Faculdade de Medicina Veterinária de Araçatuba, Brasil.; LEONIDAS CARRASCO-LETELIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CATALINA PICASSO-RISSO, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay./Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.; BRUNA NICOLETI SANTANA, Faculdade de Medicina Veterinária, Universidade Estadual Paulista, Araçatuba, Brazil.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Dairy calves in Uruguay are reservoirs of zoonotic subtypes of cryptosporidium parvum and Pose a potential risk of surface water contamination. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Frontiers in Veterinary Science, July 2020, v.7, 562. OPEN ACCESS. DOI:https://doi.org/10.3389/fvets.2020.00562 |
DOI : |
10.3389/fvets.2020.00562 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 20 March 2020/Accepted: 15 July 2020/Published: 21 August 2020.*Correspondence: Rubén Darío Caffarena
(dcaffarena@inia.org.uy) Federico Giannitti (fgiannitti@inia.org.uy). This work was partially funded by grants FMV_1_2014_1_104922, of the Uruguayan Agencia Nacional de Investigación e Innovación (ANII), and grants N-15156_PL_15_0_00 and N-23398 of the Instituto
Nacional de Investigación Agropecuaria (INIA). RC acknowledges support from ANII through a master?s scholarship (POS_FMV_2015_1_1005180). |
Contenido : |
Abstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have zoonotic potential. Subtype IIaA15G2R1 was the most frequent (53.6%; 89/166), followed by IIaA20G1R1 (24.1%; 40/166), IIaA22G1R1 (11.4%; 19/166), IIaA23G1R1 (3.6%; 6/166), IIaA17G2R1 (3%; 5/166), IIaA21G1R1 (2.4%; 4/166), and IIaA16G1R1 (1.8%; 3/166). There were no significant differences in the proportions of diarrheic and non-diarrheic calves infected with any of the C. parvum subtypes. Two spatial clusters were detected, one of which overlapped with Uruguay?s capital city and its main water potabilization plant (Aguas Corrientes), harvesting surface water to supply ~1,700,000 people. Infected calves on all farms were within 20?900 m of a natural surface watercourse draining the farmland, 10 of which flowed into six water potabilization plants located 9?108 km downstream. Four watercourses flowed downstream into Aguas Corrientes. Calves are reservoirs of zoonotic C. parvum subtypes in Uruguay and pose a public health risk. MenosAbstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have ... Presentar Todo |
Palabras claves : |
BOVINE CRYPTOSPORIDIOSIS,; CRYPTOSPORIDIUM PARVUM ZOONOTIC SUBTYPES; DAIRY CALVES; DIARRHEA; PLATAFORMA DE SALUD ANIMAL; SPATIAL CLUSTERS; SURFACE WATER; TERNEROS LECHEROS. |
Thesagro : |
URUGUAY. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16689/1/fvets-07-00562.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473207/pdf/fvets-07-00562.pdf
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Marc : |
LEADER 04044naa a2200325 a 4500 001 1061297 005 2022-09-05 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3389/fvets.2020.00562$2DOI 100 1 $aCAFFARENA, D. 245 $aDairy calves in Uruguay are reservoirs of zoonotic subtypes of cryptosporidium parvum and Pose a potential risk of surface water contamination.$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 20 March 2020/Accepted: 15 July 2020/Published: 21 August 2020.*Correspondence: Rubén Darío Caffarena (dcaffarena@inia.org.uy) Federico Giannitti (fgiannitti@inia.org.uy). This work was partially funded by grants FMV_1_2014_1_104922, of the Uruguayan Agencia Nacional de Investigación e Innovación (ANII), and grants N-15156_PL_15_0_00 and N-23398 of the Instituto Nacional de Investigación Agropecuaria (INIA). RC acknowledges support from ANII through a master?s scholarship (POS_FMV_2015_1_1005180). 520 $aAbstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have zoonotic potential. Subtype IIaA15G2R1 was the most frequent (53.6%; 89/166), followed by IIaA20G1R1 (24.1%; 40/166), IIaA22G1R1 (11.4%; 19/166), IIaA23G1R1 (3.6%; 6/166), IIaA17G2R1 (3%; 5/166), IIaA21G1R1 (2.4%; 4/166), and IIaA16G1R1 (1.8%; 3/166). There were no significant differences in the proportions of diarrheic and non-diarrheic calves infected with any of the C. parvum subtypes. Two spatial clusters were detected, one of which overlapped with Uruguay?s capital city and its main water potabilization plant (Aguas Corrientes), harvesting surface water to supply ~1,700,000 people. Infected calves on all farms were within 20?900 m of a natural surface watercourse draining the farmland, 10 of which flowed into six water potabilization plants located 9?108 km downstream. Four watercourses flowed downstream into Aguas Corrientes. Calves are reservoirs of zoonotic C. parvum subtypes in Uruguay and pose a public health risk. 650 $aURUGUAY 653 $aBOVINE CRYPTOSPORIDIOSIS, 653 $aCRYPTOSPORIDIUM PARVUM ZOONOTIC SUBTYPES 653 $aDAIRY CALVES 653 $aDIARRHEA 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aSPATIAL CLUSTERS 653 $aSURFACE WATER 653 $aTERNEROS LECHEROS 700 1 $aMEIRELES, M.V 700 1 $aCARRASCO-LETELIER, L. 700 1 $aPICASSO-RISSO, C. 700 1 $aSANTANA ,BN 700 1 $aRIET-CORREA, F. 700 1 $aGIANNITTI, F. 773 $tFrontiers in Veterinary Science, July 2020$gv.7, 562. OPEN ACCESS. DOI:https://doi.org/10.3389/fvets.2020.00562
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
28/05/2019 |
Actualizado : |
05/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
KASPARY, T. E.; CUTTI, L.; BELLÉ, C.; CASAROTTO, G.; GROTH, M.Z; DA SILVA, G.B.P.; DE AGUIAR, A.C.M. |
Afiliación : |
TIAGO EDU KASPARY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUAN CUTTI, Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Fitotecnia, 91540-000 Porto Alegre, Rio Grande do Sul, Brazil.; CRISTIANO BELLÉ, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Solos, 97105-900, Santa Maria, Rio Grande do Sul, Brazil.; GABRIELE CASAROTTO, Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Fitotecnia, 91540-000 Porto Alegre, Rio Grande do Sul, Brazil.; MÁRTIN ZANCHETT GROTH, Universidade Federal de Pelotas, Pós-Graduação em Ciência e Tecnologia de Sementes, Campus Capão do Leão s/n, Pelotas, Rio Grande do Sul, 96001-970, Brazil.; GERARDA BEATRIZ PINTO DA SILVA, Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Fitotecnia, Porto Alegre, Rio Grande do Sul, 91540-000, Brazil; ADALIN CEZAR MORAES DE AGUIAR, Universidade Federal de Viçosa, Programa de Pós-Graduação em Fitotecnia, Campus Universitário, Viçosa, Minas Gerais, 36570 900, Brazil. |
Título : |
Non-destructive analysis of photosynthetic pigments in Avena strigosa and Avena sative. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Australian Journal of Crop Science, 1 March 2019. volume 13, Issue 3, , Pages 354-359 [Open Access]. |
ISSN : |
1835-2707 |
DOI : |
10.21475/ajcs.19.13.03.p1149 |
Idioma : |
Inglés |
Contenido : |
Abstract:
Chlorophylls and carotenoids are the main photosynthetic pigments in plants. The photosynthetic potential of crop plants is used to determine the correct rate of nitrogen fertilization. To date, no studies have been conducted to understand the relationship between different methods of measurement of photosynthetic pigments in oats. The objective of this study was to quantify and compare the levels of photosynthetic pigments in Avena strigosa and A. sativa using two different methods, the extraction method and portable chlorophyll meter, and to determine whether the results of these two methods showed a significant correlation. Photosynthetic pigments were measured using both methods in a greenhouse and the laboratory at four developmental stages: tillering [28 days after sowing (DAS)], vegetative stage I (55 DAS), vegetative stage II (75 DAS), and reproductive stage (120 DAS). The same leaves were used to measure the relative chlorophyll content using a portable chlorophyll meter and extractable chlorophyll using the laboratory extraction method. The readings of the chlorophyll index differed for each developmental stage of both A. sativa and A. strigosa. The contents of chlorophyll a, chlorophyll b, and carotenoids determined using the extraction method showed high coefficients of correlation with the total chlorophyll index determined using the portable chlorophyll meter. Thus, the measurement of chlorophyll using the portable chlorophyll meter can be used for the accurate evaluation of the photosynthetic potential of oats, thus saving time and reagents. MenosAbstract:
Chlorophylls and carotenoids are the main photosynthetic pigments in plants. The photosynthetic potential of crop plants is used to determine the correct rate of nitrogen fertilization. To date, no studies have been conducted to understand the relationship between different methods of measurement of photosynthetic pigments in oats. The objective of this study was to quantify and compare the levels of photosynthetic pigments in Avena strigosa and A. sativa using two different methods, the extraction method and portable chlorophyll meter, and to determine whether the results of these two methods showed a significant correlation. Photosynthetic pigments were measured using both methods in a greenhouse and the laboratory at four developmental stages: tillering [28 days after sowing (DAS)], vegetative stage I (55 DAS), vegetative stage II (75 DAS), and reproductive stage (120 DAS). The same leaves were used to measure the relative chlorophyll content using a portable chlorophyll meter and extractable chlorophyll using the laboratory extraction method. The readings of the chlorophyll index differed for each developmental stage of both A. sativa and A. strigosa. The contents of chlorophyll a, chlorophyll b, and carotenoids determined using the extraction method showed high coefficients of correlation with the total chlorophyll index determined using the portable chlorophyll meter. Thus, the measurement of chlorophyll using the portable chlorophyll meter can be used for th... Presentar Todo |
Palabras claves : |
AVENA STRIGOSA SCHREB; CAROTENOIDS; CHLOROPHYLL; PORTABLE CHLOROPHYLL. |
Thesagro : |
AVENA SATIVA. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12712/1/Kaspary-TE.-2019.-Australian-Jr.-Crop-Science.-Non-destructiveanalysisofphotosyntheticpigmentsinAvenastrigosaandAvenasativa.pdf
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Marc : |
LEADER 02441naa a2200277 a 4500 001 1059785 005 2022-09-05 008 2019 bl uuuu u00u1 u #d 022 $a1835-2707 024 7 $a10.21475/ajcs.19.13.03.p1149$2DOI 100 1 $aKASPARY, T. E. 245 $aNon-destructive analysis of photosynthetic pigments in Avena strigosa and Avena sative.$h[electronic resource] 260 $c2019 520 $aAbstract: Chlorophylls and carotenoids are the main photosynthetic pigments in plants. The photosynthetic potential of crop plants is used to determine the correct rate of nitrogen fertilization. To date, no studies have been conducted to understand the relationship between different methods of measurement of photosynthetic pigments in oats. The objective of this study was to quantify and compare the levels of photosynthetic pigments in Avena strigosa and A. sativa using two different methods, the extraction method and portable chlorophyll meter, and to determine whether the results of these two methods showed a significant correlation. Photosynthetic pigments were measured using both methods in a greenhouse and the laboratory at four developmental stages: tillering [28 days after sowing (DAS)], vegetative stage I (55 DAS), vegetative stage II (75 DAS), and reproductive stage (120 DAS). The same leaves were used to measure the relative chlorophyll content using a portable chlorophyll meter and extractable chlorophyll using the laboratory extraction method. The readings of the chlorophyll index differed for each developmental stage of both A. sativa and A. strigosa. The contents of chlorophyll a, chlorophyll b, and carotenoids determined using the extraction method showed high coefficients of correlation with the total chlorophyll index determined using the portable chlorophyll meter. Thus, the measurement of chlorophyll using the portable chlorophyll meter can be used for the accurate evaluation of the photosynthetic potential of oats, thus saving time and reagents. 650 $aAVENA SATIVA 653 $aAVENA STRIGOSA SCHREB 653 $aCAROTENOIDS 653 $aCHLOROPHYLL 653 $aPORTABLE CHLOROPHYLL 700 1 $aCUTTI, L. 700 1 $aBELLÉ, C. 700 1 $aCASAROTTO, G. 700 1 $aGROTH, M.Z 700 1 $aDA SILVA, G.B.P. 700 1 $aDE AGUIAR, A.C.M. 773 $tAustralian Journal of Crop Science, 1 March 2019. volume 13, Issue 3, , Pages 354-359 [Open Access].
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